Expression of Transcription Factors of Rice Flag Leaf under Low Nitrogen Stress

doi:10.3969/j.issn.10017216.2012.03.004

Abstract

Abstract: The expression changes of transcription factor genes in two rice cultivars with different chlorophyll content were analyzed under low nitrogen stress by the Agilent rice genome array. The results showed that the expression levels of 53 transcription factor genes (35 downregulated genes and 18 upregulated at the transcription level) in flag leaf of supergreen rice Shennong 196(SN196) and 27 (21 downregulated and 6 upregulated at the transcription level) in flag leaves of Toyonishiki were affected by low nitrogen stress.48 genes from SN196 and 22 from Toyonishiki were cultivarspecial in response to low nitrogen stress.There were five transcription factor genes in response to low nitrogen stress in SN196 and Toyonishiki, with 1 upregulated and 4 downregulated at the transcription level, respectively. Expression of transcription factor genes were affected by low nitrogen stress in flag leaf, and there were genes of cultivar specialresponse as well as overlap between SN196 and Toyonishiki, respectively.Distribution of low nitrogen stress regulating genes on the rice chromosomes of the two cultivars was different.

Key words: rice, flag leaf, microarray, transcription factor

摘要： 利用Agilent 4×44K芯片全基因组研究低氮胁迫下，2个不同叶绿素含量水稻齐穗期剑叶的转录因子相关基因表达的变化。结果表明，低氮处理与对照相比，超绿水稻沈农196（SN196）剑叶共有53个转录因子相关基因表达发生变化（35个在转录水平下调表达，18个在转录水平上调表达）。丰锦剑叶有27个转录因子相关基因表达发生变化（21个在转录水平下调表达，6个在转录水平上调表达）。低氮胁迫响应转录因子相关基因表现出品种（系）特异性，超绿水稻SN196有48个特异响应，丰锦有22个特异响应。两个水稻品种（系）低氮胁迫响应的转录因子相关基因有5个重叠，其中1个在转录水平上调表达，4个下调表达。低氮胁迫下，水稻剑叶转录因子相关基因的表达发生变化，不同叶绿素含量水稻品种（系）既表现特异性，也存在部分重叠。两个水稻的低氮胁迫响应转录因子基因在染色体上的分布存在差异。

关键词: 水稻, 剑叶, 基因芯片, 转录因子

CLC Number:

ZHAO Minghui, MA Dianrong, WANG Jiayu, XU Hai, TANG Liang, CHEN Wenfu. Expression of Transcription Factors of Rice Flag Leaf under Low Nitrogen Stress[J]. Chinese Journal of Rice Science, 2012, 26(3): 275-282.

赵明辉,马殿荣,王嘉宇,徐海,唐亮,陈温福. 低氮胁迫下水稻剑叶转录因子表达变化[J]. 中国水稻科学, 2012, 26(3): 275-282.

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